Coding
Part:BBa_K3286200:Design
Designed by: Ben Kuipers Group: iGEM19_Wageningen_UR (2019-10-19)
RpfCch
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1861
Illegal BglII site found at 227 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1861
Illegal PstI site found at 826
Illegal PstI site found at 1432
Illegal PstI site found at 2059
Illegal NgoMIV site found at 757
Illegal NgoMIV site found at 848
Illegal NgoMIV site found at 1675
Illegal NgoMIV site found at 1770 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Non codon optimized version
Source
Derived from a paper