Coding

Part:BBa_K3286200:Design

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-19)


RpfCch


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1861
    Illegal BglII site found at 227
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 826
    Illegal PstI site found at 1432
    Illegal PstI site found at 2059
    Illegal NgoMIV site found at 757
    Illegal NgoMIV site found at 848
    Illegal NgoMIV site found at 1675
    Illegal NgoMIV site found at 1770
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Non codon optimized version


Source

Derived from a paper

References